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Goat Anti-DDB1 Antibody (EB05033)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB05033 | Goat Anti-DDB1 Antibody | Pep-ELISA, WB |  |
100µg specific antibody in 200µl | Human, Mouse, Rat, Dog, Cow | $315.00 |  |
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Ordering Information forOrder direct from Everest
$40.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Order offlineNow shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:
In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day. Orders are placed with and invoiced by our US partner Abcore LLC.
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Target Protein
Principal Names: XPE, damage-specific DNA binding protein 1 (127kD), DDB p127 subunit, UV-DDB1, XPE-BF, XPCE, XAP1, DDBA, damage-specific DNA binding protein 1, 127kDa, DDB1Official Symbol: DDB1
Accession Number(s): NP_001914.3
Human GeneID(s): 1642
Non-Human GeneID(s): 64470 (rat)
Immunogen
Peptide with sequence C-DLIKVVEELTRIH, from the C Terminus of the protein sequence according to NP_001914.3.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Approx 130kDa band seen in HeLa, Jurkat and NSO (mouse) cell lysates. Recommended for use at 0.5-1.5 µg/ml.
Species Reactivity
Tested: Human, MouseExpected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Specific References
This antibody has been successfully used in the following papers:
Li T, Robert EI, van Breugel PC, Strubin M, Zheng N.
A promiscuous alpha-helical motif anchors viral hijackers and substrate receptors to the CUL4-DDB1 ubiquitin ligase machinery.
Nat Struct Mol Biol. 2010 Jan;17(1):105-11.
PMID: 19966799
The goat polyclonal antibody used in the following papers was manufactured by us:
Westbrook TF, Martin ES, Schlabach MR, Leng Y, Liang AC, Feng B, Zhao JJ, Roberts TM, Mandel G, Hannon GJ, Depinho RA, Chin L, Elledge SJ.
A genetic screen for candidate tumor suppressors identifies REST.
Cell. 2005 Jun 17;121(6):837-48.
PMID: 15960972
Leung-Pineda V, Huh J, Piwnica-Worms H.
DDB1 targets Chk1 to the Cul4 E3 ligase complex in normal cycling cells and in cells experiencing replication stress
Cancer Res. 2009 Mar 15;69(6):2630-7.
PMID: 19276361
Alekseev S, Luijsterburg MS, Pines A, Geverts B, Mari PO, Giglia-Mari G, Lans H, Houtsmuller AB, Mullenders LH, Hoeijmakers JH, Vermeulen W.
Cellular concentrations of DDB2 regulate dynamic binding of DDB1 at UV-induced DNA damage.
Mol Cell Biol. 2008 Dec;28(24):7402-13.
PMID: 18936169
Luijsterburg MS, Goedhart J, Moser J, Kool H, Geverts B, Houtsmuller AB, Mullenders LH, Vermeulen W, van Driel R.
Dynamic in vivo interaction of DDB2 E3 ubiquitin ligase with UV-damaged DNA is independent of damage-recognition protein XPC.
J Cell Sci. 2007 Aug 1;120(Pt 15):2706-16.
PMID: 17635991