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Goat Anti-DDX58 Antibody (EB08003)

Code Name Applications Availability Size Tested species Price Grade  
EB08003 Goat Anti-DDX58 Antibody Pep-ELISA, FC, IF, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Ph. (800) 832-2611
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Email: info@arp1.com
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EB08003 1
EB08003 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB08003 2
EB08003 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB08003 3
EB08003 Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB08003 4
EB08003 (3.75µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08003 5
EB08003 (3.75µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08003 6
EB08003 (3.75µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: DDX58, DEAD (Asp-Glu-Ala-Asp) box polypeptide 58, DKFZp434J1111, DKFZp686N19181, FLJ13599, RIG-I, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide RIG-I, OTTHUMP00000045225, RNA helicase, RNA helicase RIG-I, retinoic acid-inducible gene I
Official Symbol: DDX58
Accession Number(s): NP_055129.2
Human GeneID(s): 23586

Immunogen

Peptide with sequence C-KDFHFEKIPFDPAE, from the C Terminus of the protein sequence according to NP_055129.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
IHC: Paraffin embedded Human Spleen, Placenta, and Brain (Cortex). Recommended concentration: 3.75µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of U937 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse

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