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Goat Anti-Delta-Sarcoglycan (aa245-257) Antibody (EB10758)

Related Products: EBP10758-B - Immunizing Peptide, EBP10758 - Immunizing Peptide, Goat Anti-Delta-Sarcoglycan (aa245-257), Biotinylated Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10758 Goat Anti-Delta-Sarcoglycan (aa245-257) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10758 1
EB10758 (0.1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB10758 2
EB10758 (3.8µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SGCD, sarcoglycan, delta (35kDa dystrophin-associated glycoprotein), 35DAG, CMD1L, DAGD, MGC22567, SG-delta, SGCDP, SGD, 35kD dystrophin-associated glycoprotein, delta-SG, delta-sarcoglycan, dystrophin associated
Official Symbol: SGCD
Accession Number(s): NP_000328.2; NP_758447.1; NP_001121681.1
Human GeneID(s): 6444
Non-Human GeneID(s): 24052 (mouse), 497892 (rat)
Important Comments: This antibody is expected to recognize isoform 1 (NP_000328.2) and isoform 3 (NP_001121681.1).

Immunogen

Peptide with sequence C-RLPHGSYTPTGTR, from the internal region of the protein sequence according to NP_000328.2; NP_758447.1; NP_001121681.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 37kDa band observed in Human and Rat Heart lysates (calculated MW of 32.2kDa according to NP_000328.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3µg/ml.
IHC: In paraffin embedded Human Skeletal Muscle shows sarcolemmar staining of the fibres. Recommended concentration, 3-5µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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