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Goat Anti-Desmoglein 1 / DSG1 Antibody (EB09586)

Related Products: EBP09586 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09586 Goat Anti-Desmoglein 1 / DSG1 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09586 1
EB09586 (3.8µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: DSG1, desmoglein 1, CDHF4, DG1, DSG, desmosomal glycoprotein 1, pemphigus foliaceus antigen
Official Symbol: DSG1
Accession Number(s): NP_001933.2
Human GeneID(s): 1828

Immunogen

Peptide with sequence C-YTTSDTLKPSVHVHD, from the internal region of the protein sequence according to NP_001933.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments in lysates of cell lines A431, HeLa, K562 and MOLT4 gave no specific signal but low background (at antibody concentration up to 1µg/ml).
IHC: In paraffin embedded Human Skin showsstaining of the cellular membranes of keratinocytes. Recommended concentration, 4-6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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