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Goat Anti-DGAT2 Antibody (EB07661)

Related Products: EBP07661 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB07661 Goat Anti-DGAT2 Antibody Pep-ELISA, IHC, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog $315.00 Elite

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EB07661 1
EB07661 (0.5µg/ml) staining of Human Liver lysate in the third lane (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07661 2
HEK293 overexpressing DGAT2 (lane A) and probed with EB07661 (mock transfection in lane B).
EB07661 3
EB07661 (3µg/ml) staining of paraffin embedded Human Liver. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: diacylglycerol O-acyltransferase-like protein 2 isoform 1, diacylglycerol O-acyltransferase homolog 2, GS1999full, HMFN1045, DKFZp686A15125, diacylglycerol O-acyltransferase homolog 2 (mouse), DGAT2
Official Symbol: DGAT2
Accession Number(s): NP_115953.2
Human GeneID(s): 84649
Non-Human GeneID(s): 67800 (mouse), 252900 (rat)
Important Comments: This antibody is not expected to cross-react with other DGAT members.

Immunogen

Peptide with sequence C-KLEHPTQQDIDLYH, from the internal region (near the C Terminus) of the protein sequence according to NP_115953.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: In transfected HEK293 transiently expressing DGAT2 bands of approx. 60kDa and 45kDa are observed. These bands are not observed in the non-transfected HEK293. Similar results are observed in Human Liver lysates. The calculated molecular size is 43.8kDa according to NP_115953.2. Recommended concentration, 0.3-1µg/ml.
IHC: In paraffin embedded Human Liver shows granular cytoplasm staining of hepatocytes. Recommended concentration, 3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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