Change country
Tel: 888-320-GOAT (4628)
Fax: 888-841-9041
Home

Goat Anti-DGAT2 Antibody (EB07661)

Related Products: EBP07661 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB07661 Goat Anti-DGAT2 Antibody ELISA, IHC, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog $300.00 Elite

Ordering Information for

Order direct from Everest

$35.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA.

Order offline

Now shipping direct to US customers! All orders will be shipped to you by our logistics partner in Kentucky. Almost all products are held in stock for immediate shipment, see individual product pages for availability. Orders are placed with and invoiced by Everest Biotech Ltd in the United Kingdom.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (sales@everestbiotech.com), fax (888-841-9041), phone (888-320-GOAT) or mail. Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 09.30 PST / 12.30 EST on Monday through Thursday are shipped the same day.

OR

Order from our local distributor

Axxora, LLC

6181 Cornerstone Court East Suite 103,
San Diego
CA 92121-4727
USA

Tel: 858-550-8830
Toll Free Tel: 1-800-550-3033
Fax: 858-550-8825
Toll Free Fax: 1-800-550-8825
Email: axxora-usa@axxora.com
Website: www.axxora.com
EB07661 1
EB07661 (0.5µg/ml) staining of Human Liver lysate in the third lane (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07661 2
HEK293 overexpressing DGAT2 (lane A) and probed with EB07661 (mock transfection in lane B).
EB07661 3
EB07661 (3µg/ml) staining of paraffin embedded Human Liver. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: diacylglycerol O-acyltransferase-like protein 2 isoform 1, diacylglycerol O-acyltransferase homolog 2, GS1999full, HMFN1045, DKFZp686A15125, diacylglycerol O-acyltransferase homolog 2 (mouse), DGAT2
Official Symbol: DGAT2
Accession Number(s): NP_115953.2
Human GeneID(s): 84649
Non-Human GeneID(s): 67800 (mouse), 252900 (rat)
Important Comments: This antibody is not expected to cross-react with other DGAT members.

Immunogen

Peptide with sequence C-KLEHPTQQDIDLYH, from the internal region (near the C Terminus) of the protein sequence according to NP_115953.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: In transfected HEK293 transiently expressing DGAT2 bands of approx. 60kDa and 45kDa are observed. These bands are not observed in the non-transfected HEK293. Similar results are observed in Human Liver lysates. The calculated molecular size is 43.8kDa according to NP_115953.2. Recommended concentration, 0.3-1µg/ml.
IHC: In paraffin embedded Human Liver shows granular cytoplasm staining of hepatocytes. Recommended concentration, 3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

Background Reference

Cases S, Stone SJ, Zhou P, Yen E, Tow B, Lardizabal KD, Voelker T, Farese RV Jr.
Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase, and related family members.
J Biol Chem. 2001 Oct 19;276(42):38870-6. Epub 2001 Jul 31.
PMID: 11481335

Product Reviews

Please login to review this product.