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Goat Anti-DGCR8 / Pasha Antibody (EB09141)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB09141 | Goat Anti-DGCR8 / Pasha Antibody | Pep-ELISA |  |
100µg specific antibody in 200µl | Human, Mouse, Rat, Cow | $210.00 |  |
|
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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day. Orders are placed with and invoiced by our US partner Abcore LLC.
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Target Protein
Principal Names: pasha, Gy1, DGCRK6, C22orf12, DiGeorge syndrome critical region gene 8, DGCR8Official Symbol: DGCR8
Accession Number(s): NP_073557.3
Human GeneID(s): 54487
Non-Human GeneID(s): 94223 (mouse), 287954 (rat)
Immunogen
Peptide with sequence C-KRFDFEQVTVKKFRT, from the internal region of the protein sequence according to NP_073557.3.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Preliminary experiments gave an approx. 75kDa band in Human Brain (amygdala) lysates after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 86.0kDa according to NP_073557.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Species Reactivity
Tested:Expected from sequence similarity: Human, Mouse, Rat, Cow