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Goat Anti-DKK1 Antibody (EB06457)

Related Products: EBP05358 - Immunizing Peptide, EBP06457 - Immunizing Peptide, Goat Anti-DKK1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06457 Goat Anti-DKK1 Antibody Pep-ELISA, WB, IF, IHC, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06457 1
EB06457 (1µg/ml) staining of MCF7 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06457 2
EB06457 (10µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06457 3
EB06457 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06457 4
EB06457 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: DKK1, SK, DKK-1, dickkopf homolog 1 (Xenopus laevis), dickkopf-1 like, dickkopf (Xenopus laevis) homolog 1, dickkopf homolog 1, dickkopf related protein-1
Official Symbol: DKK1
Accession Number(s): NP_036374.1
Human GeneID(s): 22943

Immunogen

Peptide with sequence CDHHQASNSSRLHT, from the C Terminus of the protein sequence according to NP_036374.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 38kDa band observed in lysates of cell line MCF7 and in preliminary testing of HepG2 cell lysate and Human Spleen lysate (calculated MW of 28.7kDa accordin to NP_036374.1). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Placenta. Recommended concentration: 10µg/ml.

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HepG2 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Cow

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