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Goat Anti-DLX5 Antibody (EB09284)

Related Products: EBP09284 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09284 Goat Anti-DLX5 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09284 1
EB09284 (1µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09284 2
EB09284 (2.5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: DLX5, distal-less homeobox 5, distal-less homeo box 5
Official Symbol: DLX5
Accession Number(s): NP_005212.1
Human GeneID(s): 1749
Non-Human GeneID(s): 13395 (mouse), 25431 (rat)

Immunogen

Peptide with sequence C-AYNRVPSATNQPEK, from the internal region of the protein sequence according to NP_005212.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 30kDa band observed in Human Bone Marrow lysates (calculated MW of 31.5kDa according to NP_005212.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lee et al, J Biol Chem. 2003 Sep 5;278(36):34387-94; PMID: 12815054). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Placenta. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

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