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Goat Anti-Duffy / FY / DARC Antibody (EB06940)

Related Products: EBP06940 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB06940 Goat Anti-Duffy / FY / DARC Antibody Pep-ELISA, WB, IHC, FC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB06940 1
EB06940 (0.1µg/ml) staining of human liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06940 2
EB06940 (3µg/ml) staining of paraffin embedded human kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: OTTHUMP00000035294, OTTHUMP00000035293, Fy glycoprotein, WBCQ1, GpFy, Dfy, Glycoprotein D, Duffy blood group antigen, Duffy blood group, chemokine receptor, OTTHUMP00000060081, FY, CD234, CTA-134P22.3, GPD, CCBP1, Duffy blood group, HGNC:4035, DARC, FY
Official Symbol: DARC
Accession Number(s): NP_001116423.1; NP_002027.2
Human GeneID(s): 2532
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001116423.1; NP_002027.2).

Immunogen

Peptide with sequence HRAELSPSTENSSQLDFED-C, from the N Terminus of the protein sequence according to NP_001116423.1; NP_002027.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 35kDa band observed in human kidney and human liver lysates (calculated MW of 35.6kDa according to NP_002027.2). Recommended concentration: 0.1-0.3µg/ml.
IHC: In paraffin embedded human kidney, stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. This antibody has also been successfully used on Huamn Lung showing endothelial staining. Recommended concentration, 3µg/ml.

Flow cytometry: A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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