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Goat Anti-DUSP14 / MKP6 Antibody (EB05320)

Code Name Applications Availability Size Species reactivity Price Grade  
EB05320 Goat Anti-DUSP14 / MKP6 Antibody Pep-ELISA Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Cow $210.00 Aspiring

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Target Protein

Principal Names: OTTHUMP00000164065, OTTHUMP00000164064, MKP-1 like protein tyrosine phosphatase, MKP-L, MKP6, dual specificity phosphatase 14, DUSP14
Official Symbol: DUSP14
Accession Number(s): NP_008957.1
Human GeneID(s): 11072
Non-Human GeneID(s): 56405 (mouse)

Immunogen

Peptide with sequence CEKESRHLMPYWGI, from the C Terminus of the protein sequence according to NP_008957.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: No signal obtained yet but low background observed in Human Liver extracts at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Species Reactivity

Tested:
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

The goat polyclonal antibody used in this paper was manufactured by us:
Zhu Y, Wang C, Huang J, Ge Z, Dong Q, Zhong X, Su Y, Zheng S.
The Helicobacter pylori virulence factor CagA promotes Erk1/2-mediated Bad phosphorylation in lymphocytes: a mechanism of CagA-inhibited lymphocyte apoptosis.
Cell Microbiol.2007 Apr;9(4):952-61.
PMID: 17140404

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