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Goat Anti-DUSP8 / HVH5 Antibody (EB05318)

Related Products: EBP05318 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB05318 Goat Anti-DUSP8 / HVH5 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Cow $315.00 Elite

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EB05318 1
EB05318 staining (2µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: OTTHUMP00000164487, FLJ42958, FLJ42476, C11orf81, serine/threonine specific protein phosphatase, H1 phosphatase, vaccinia virus homolog, HVH-5, HVH8, HB5, dual specificity phosphatase 8, DUSP8
Official Symbol: DUSP8
Accession Number(s): NP_004411.2
Human GeneID(s): 1850
Non-Human GeneID(s): 18218 (mouse)
Important Comments: This antibody is also expected to recognise the hypothetical human protein similar to dual specificity phosphatase 8 (XM_114902), which is virtually identical.

Immunogen

Peptide with sequence *AGDRLPRKVMDAK-C, from the N Terminus of the protein sequence according to NP_004411.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 60-65kDa bands seen in Human Heart lysate. Recommended for use at 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

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