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Goat Anti-EAT2 phospho (Y127) Antibody (EB03002)

Code Name Applications Availability Size Tested species Price Grade  
EB03002 Goat Anti-EAT2 phospho (Y127) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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Target Protein

Principal Names: SH2D1B, SH2 domain containing 1B, EAT2, SH2 domain-containing molecule EAT2
Official Symbol: SH2D1B
Accession Number(s): NP_444512.2
Human GeneID(s): 117157


Phosphorylated peptide with sequence C-NSNSDpYVDVLP, from the internal region of the protein sequence according to NP_444512.2

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 14kDa band observed in lysates of cell line A431 (calculated MW of 16.6kDa according to NP_002643.1). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific Reference

This antibody has been successfully used in Western blot:
Clarkson NG, Simmonds SJ, Puklavec MJ, Brown MH.
Direct and indirect interactions of the cytoplasmic region of CD244 (2B4) in mice and humans with FYN kinase.
J Biol Chem. 2007 Aug 31;282(35):25385-94.
PMID: 17599905

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