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Goat Anti-EMI2 Antibody (EB06061)

Code Name Applications Availability Size Species reactivity Price Grade  
EB06061 Goat Anti-EMI2 Antibody Pep-ELISA, WB, FC, ICC, IF Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Pig, Cow $315.00 Elite

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Orders are placed with and invoiced by our US partner Abcore LLC.

 

Target Protein

Principal Names: ERP1, early mitotic inhibitor 2, Fbx43, F-box protein 43, EMI2, FBXO43
Official Symbol: FBXO43
Accession Number(s): NP_001025031.2
Human GeneID(s): 286151
Non-Human GeneID(s): 78803 (mouse), 315034 (rat)

Immunogen

Peptide with sequence C-PGSAQSKRNLKRL, from the C Terminus of the protein sequence according to NP_001025031.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: This product has been successfully used on Rat Pachytene Spermatocytes showing a 71kDa band (Perrard et al, PLoS One. 2009 Oct 5;4(10):e7237. PMID: 19802389). Recommended concentration: 1-3µg/ml.

This product has been successfully used in Immunofluorescence and Flow Cytometry on Rat meiotic Testis cells: (Perrard et al, PLoS One. 2009 Oct 5;4(10):e7237. PMID: 19802389). Recommended concentration: 5-10µg/ml.

Species Reactivity

Tested: Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific Reference

This antibody has been successfully used in the following papers:
Perrard MH, Chassaing E, Montillet G, Sabido O, Durand P.
Cytostatic Factor Proteins Are Present in Male Meiotic Cells and β-Nerve Growth Factor Increases Mos Levels in Rat Late Spermatocytes.
PLoS One. 2009 Oct 5;4(10):e7237.
PMID: 19802389

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