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Goat Anti-ERG Antibody (EB07081)

Related Products: EBP07081 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB07081 Goat Anti-ERG Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

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EB07081 1
EB07081 (4µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ets-related, TMPRSS2-ERG prostate cancer specific, OTTHUMP00000208036, OTTHUMP00000115970, v-ets erythroblastosis virus E26 oncogene homolog (avian), v-ets erythroblastosis virus E26 oncogene like, v-ets avian erythroblastosis virus E26 oncogene related, transcriptional regulator ERG (transforming protein ERG), p55, erg-3, HGNC:3446, v-ets erythroblastosis virus E26 oncogene like (avian), ERG
Official Symbol: ERG
Accession Number(s): NP_891548.1; NP_004440.1; NP_001129626.1; NP_001129627.1; NP_001230358.1|
Human GeneID(s): 2078
Non-Human GeneID(s): 13876 (mouse), 170909 (rat)
Important Comments: This antibody is expected to recognise all reported isoforms (NP_891548.1; NP_004440.1; NP_001129626.1; NP_001129627.1; NP_001230358.1)

Immunogen

Peptide with sequence C-PNTRLPTSHMPSH, from the C Terminus of the protein sequence according to NP_891548.1; NP_004440.1; NP_001129626.1; NP_001129627.1; NP_001230358.1|.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments in lysates of cell lines HEK293, A431 and Jurkat gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
IHC: In paraffin embedded Human Prostate shows nuclear staining in secretory cells of the glands. Recommended concentration, 4-8µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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