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Goat Anti-ERO1-like (aa105-118) Antibody (EB10882)

Related Products: EBP10882 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10882 Goat Anti-ERO1-like (aa105-118) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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www.arp1.com
EB10882 1
EB10882 (0.3µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10882 2
EB10882 (5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ERO1L, ERO1-like (S. cerevisiae), ERO1-alpha, ERO1-L, ERO1-L-alpha, ERO1-like protein alpha, endoplasmic oxidoreductin-1-like protein, oxidoreductin-1-L-alpha
Official Symbol: ERO1A
Accession Number(s): NP_055399.1
Human GeneID(s): 30001

Immunogen

Peptide with sequence C-QSDEVPDGIKSASY, from the internal region of the protein sequence according to NP_055399.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 70kDa band observed in lysates of cell lines A431, HeLa, Jurkat (calculated MW of 54.4kDa according to NP_055399.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Small Intestine. Recommended concentration: 5µg/ml.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371

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