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Goat Anti-ESRRG Antibody (EB08367)

Related Products: EBP08367 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08367 Goat Anti-ESRRG Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB08367 1
EB08367 (0.2µg/ml) staining of Human Breast lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB08367 2
HEK293 overexpressing ESRRG (RC218233) and probed with EB08367 (mock transfection in first lane), tested by Origene.
Click here to download PDF datasheet.

Target Protein

Principal Names: ESRRG, DKFZp781L1617, Estrogen-related receptor gamma, ERR3, FLJ16023, KIAA0832, NR3B3
Official Symbol: ESRRG
Accession Number(s): NP_001429.2; NP_996317.1; NP_996318.1
Human GeneID(s): 2104
Non-Human GeneID(s): 26381 (mouse)
Important Comments: This antibody is expected to recognise the reported isoform NP_001429.2.

Immunogen

Peptide with sequence CRMSNKDRHIDS, from the internal region of the protein sequence according to NP_001429.2; NP_996317.1; NP_996318.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 50kDa band observed in Human Brain (Cerebellum) and Breast lysates (calculated MW of 51.3kDa according to NP_NP_001429.2). In transfected HEK293 transiently expressing ESRRG a band of approx. 52kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.2-0.6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog

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