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Goat Anti-FBXO4 Antibody (EB09905)

Related Products: EBP09905 - Immunizing Peptide, EBP09906 - Immunizing Peptide, Goat Anti-FBXO4 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB09905 Goat Anti-FBXO4 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09905 1
EB09905 (2.5µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: DKFZp547N213, F-box only protein 4, F-box protein 4, F-box protein Fbx4, FBX4, FLJ10141, FBXO4
Official Symbol: FBXO4
Accession Number(s): NP_036308.1; NP_277019.1
Human GeneID(s): 26272
Non-Human GeneID(s): 106052 (mouse), 310363 (rat)
Important Comments: This antibody is expected to recognize isoform 1 and 2 (NP_036308.1; NP_277019.1).

Immunogen

Peptide with sequence C-EHTSAVNKMFSRH, from the internal region of the protein sequence according to NP_036308.1; NP_277019.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments gave an approx 48kDa band in Human Heart ysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 44.1kDa according to NP_036308.1. The 48kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Tonsil. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

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