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Goat Anti-FLVCR Antibody (EB06563)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB06563 | Goat Anti-FLVCR Antibody | Pep-ELISA, WB |  |
100µg specific antibody in 200µl | Human | $315.00 |  |
|
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Target Protein
Principal Names: FLVCR protein, feline leukemia virus subgroup C cellular receptor, FLVCROfficial Symbol: FLVCR1
Accession Number(s): NP_054772.1
Human GeneID(s): 28982
Immunogen
Peptide with sequence C-KTVMLSKQSESAI, from the C Terminus of the protein sequence according to NP_054772.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:128000.Western blot: Approx 55kDa band observed in Human Bone Marrow lysates (calculated MW of 59.9kDa according to NP_054772.1). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Successfully used in WB application in the publicatioon: Rey M.A. et al. Enhanced alternative splicing of the FLVCR1 gene in Diamond Blackfan anemia disrupts FLVCR1 expression and function that are critical for erythropoiesis. Haematologica 2008 Nov;93(11):1617-26. Epub 2008 Sep 24. PMID:18815190.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human
Specific Reference
The goat polyclonal antibody used in this paper was manufactured by us:
Rey MA, Duffy SP, Brown JK, Kennedy JA, Dick JE, Dror Y, Tailor CS.
Enhanced alternative splicing of the FLVCR1 gene in Diamond Blackfan anemia disrupts FLVCR1 expression and function that are critical for erythropoiesis.
Haematologica 2008 Nov;93(11):1617-26. Epub 2008 Sep 24.
PMID: 18815190