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Code Name Applications Availability Size Tested species Price Grade  
EB05226 Goat Anti-FOXP2 (C terminus) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB05226 1
EB05226 (1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05226 2
EB05226 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: FOXP2, forkhead box P2, SPCH1, CAGH44, TNRC10, CAG repeat protein 44, speech and language disorder 1, trinucleotide repeat containing 10, forkhead/winged-helix transcription factor, DKFZp686H1726, OTTHUMP00000196932
Official Symbol: FOXP2
Accession Number(s): NP_055306.1; NP_683696.2; NP_683697.1
Human GeneID(s): 93986
Important Comments: This antibody is expected to recognise all three reported isoforms (NP_055306.1; NP_683696.2; NP_683697.1).

Immunogen

Peptide with sequence C-REIEEEPLSEDLE, from the C Terminus of the protein sequence according to NP_055306.1; NP_683696.2; NP_683697.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 80kDa band observed in Human Brain (Cerebellum) lysate (calculated MW of 79.9kDa according to NP_055306.1). Recommended concentration: 0.5-1.5µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific References

This antibody (previous batch) has been successfully used in IHC on Human and Mouse:
Haldipur P, Dang D, Aldinger KA, Janson OK, Guimiot F, Adle-Biasette H, Dobyns WB, Siebert JR, Russo R, Millen KJ.
Phenotypic outcomes in Mouse and Human Foxc1 dependent Dandy-Walker cerebellarmal formation suggest shared mechanisms.
Elife. 2017 Jan 16;6. pii: e20898. doi: 10.7554/eLife.20898.
PMID: 28092268

This antibody (previous batch) has been successfully used in IHC on Mouse:
Fujita H, Sugihara I.
FoxP2 expression in the cerebellum and inferior olive: Development of the transverse stripe-shaped expression pattern in the mouse cerebellar cortex.
J Comp Neurol. 2012 Feb 15;520(3):656-77. doi: 10.1002/cne.22760.
PMID: 21935935

This antibody (previous batch) has been successfully used in IHC on Mouse:
Fujita H, Morita N, Furuichi T, Sugihara I.
Clustered fine compartmentalization of the mouse embryonic cerebellar cortex and its rearrangement into the postnatal striped configuration.
J Neurosci. 2012 Nov 7;32(45):15688-703.
PMID: 23136409

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