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Goat Anti-GAPDH (C Terminus) Loading Control Antibody (EB06377)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB06377 | Goat Anti-GAPDH (C Terminus) Loading Control Antibody | Pep-ELISA, WB, IHC |  |
100µg specific antibody in 200µl | Human, Mouse, Dog | $315.00 |  |
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Ordering Information forOrder direct from Everest
$40.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Order offlineNow shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:
In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day. Orders are placed with and invoiced by our US partner Abcore LLC.
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Target Protein
Principal Names: glyceraldehyde 3-phosphate dehydrogenase, aging-associated gene 9 protein, OTTHUMP00000174432, OTTHUMP00000174431, MGC88685, GAPD, G3PD, HGNC:4141, glyceraldehyde-3-phosphate dehydrogenase, GAPDHOfficial Symbol: GAPDH
Accession Number(s): NP_002037.2
Human GeneID(s): 2597
Important Comments: GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Immunogen
Peptide with sequence C-HQVVSSDFNSDT, from the C Terminus of the protein sequence according to NP_002037.2.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:2000.Western blot: Approx 35kDa band observed in lysates of Human Tonsil, Mouse Spleen, and cell lines HeLa, A431 and 293 (calculated MW of 36.1kDa according to NP_002037). Recommended concentration: 0.01-0.03µg/ml.
Species Reactivity
Tested: Human, Mouse, RatExpected from sequence similarity: Human, Mouse, Dog
Specific References
This antibody has been successfully used in the following papers:
Kiepe D, Van Der Pas A, Ciarmatori S, Ständker L, Schütt B, Hoeflich A, Hügel U, Oh J, Tönshoff B.
Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein 2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I.
Endocrinology. 2008 Oct;149(10):4901-11.
PMID: 18556354
The goat polyclonal antibody used in the following papers was manufactured by us:
Carr AJ, Vugler A, Lawrence J, Chen LL, Ahmado A, Chen FK, Semo M, Gias C, da Cruz L, Moore HD, Walsh J, Coffey PJ.
Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay.
Mol Vis. 2009;15:283-95. Epub 2009 Feb 6.
PMID: 19204785
Henson MA, Roberts AC, Salimi K, Vadlamudi S, Hamer RM, Gilmore JH, Jarskog LF, Philpot BD.
Developmental regulation of the NMDA receptor subunits, NR3A and NR1, in human prefrontal cortex.
Cereb Cortex. 2008 Nov;18(11):2560-73.
PMID: 18296432
Gerstner JR, Bremer QZ, Vander Heyden WM, Lavaute TM, Yin JC, Landry CF.
Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain.
PLoS One. 2008 Feb 20;3(2):e1631.
PMID: 18286188
Nguyen M, Pace AJ, Koller BH.
Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia.
J Exp Med. 2007 Jun 11;204(6):1383-93.
PMID: 17517966