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Code Name Applications Availability Size Species reactivity Price Grade  
EB06377 Goat Anti-GAPDH (C Terminus) Antibody ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Dog $300.00 Elite

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EB06377 1
EB06377 staining (0.01µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: glyceraldehyde 3-phosphate dehydrogenase, aging-associated gene 9 protein, OTTHUMP00000174432, OTTHUMP00000174431, MGC88685, GAPD, G3PD, HGNC:4141, glyceraldehyde-3-phosphate dehydrogenase, GAPDH
Official Symbol: GAPDH
Accession Number(s): NP_002037.2
Human GeneID(s): 2597
Important Comments: GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.

Immunogen

Peptide with sequence C-HQVVSSDFNSDT, from the C Terminus of the protein sequence according to NP_002037.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 35kDa band observed in lysates of Human Tonsil, Mouse Spleen, and cell lines HeLa, A431 and 293 (calculated MW of 36.1kDa according to NP_002037). Recommended concentration: 0.01-0.03µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Dog

Specific References

The goat polyclonal antibody used in this paper was manufactured by us:
Carr AJ, Vugler A, Lawrence J, Chen LL, Ahmado A, Chen FK, Semo M, Gias C, da Cruz L, Moore HD, Walsh J, Coffey PJ.
Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay.
Mol Vis. 2009;15:283-95. Epub 2009 Feb 6.
PMID: 19204785

The goat polyclonal antibody used in this paper was manufactured by us:
Kiepe D, Van Der Pas A, Ciarmatori S, Ständker L, Schütt B, Hoeflich A, Hügel U, Oh J, Tönshoff B.
Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein 2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I.
Endocrinology. 2008 Oct;149(10):4901-11. Epub 2008 Jun 12.
PMID: 18556354

The goat polyclonal antibody used in this paper was manufactured by us:
Henson MA, Roberts AC, Salimi K, Vadlamudi S, Hamer RM, Gilmore JH, Jarskog LF, Philpot BD.
Developmental regulation of the NMDA receptor subunits, NR3A and NR1, in human prefrontal cortex.
Cereb Cortex. 2008 Nov;18(11):2560-73.
PMID: 18296432

The goat polyclonal antibody used in this paper was manufactured by us:
Gerstner JR, Bremer QZ, Vander Heyden WM, Lavaute TM, Yin JC, Landry CF.
Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain.
PLoS One. 2008 Feb 20;3(2):e1631.
PMID: 18286188

The goat polyclonal antibody used in this paper was manufactured by us:
Nguyen M, Pace AJ, Koller BH.
Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia.
J Exp Med. 2007 Jun 11;204(6):1383-93.
PMID: 17517966

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