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Goat Anti-GATA3 Antibody (EB05570)

Code Name Applications Availability Size Tested species Price Grade  
EB05570 Goat Anti-GATA3 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

Ordering Information for

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$49.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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  • Purchase order number
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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US partner Abcore LLC.

 

OR

Order from our local distributor

Labscoop

Little Rock, Arkansas
P.O. Box 242164

Email: support@labscoop.com
Website: https://www.labscoop.com/
Phone: 800.316.3081
Fax: 800.316.3081

EB05570 1
EB05570 (0.5µg/ml) staining of Human Tonsil (A) and Mouse Kidney (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
EB05570 2
EB05570 (3µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. A) staining of cells in the germinal centre, B) staining of cells in the interfollicular area. This data was obtained using a previous batch.
Click here to download PDF datasheet.

Target Protein

Principal Names: GATA3, GATA binding protein 3, HDR, MGC2346, MGC5199, MGC5445, GATA-binding protein 3, enhancer-binding protein GATA3
Official Symbol: GATA3
Accession Number(s): NP_001002295.1; NP_002042.1
Human GeneID(s): 2625
Non-Human GeneID(s): 14462 (mouse)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001002295.1 and NP_002042.1).

Immunogen

Peptide with sequence EVTADQPRWVSHH-C, from the N Terminus of the protein sequence according to NP_001002295.1; NP_002042.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 46kDa band observed in Human Tonsil and in Mouse Kidney lysates. (calculated MW of 47.9kDa according to NP_002042.1 and Mouse NP_032117.1 ). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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