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Goat Anti-GFAP Antibody (EB07478)

Code Name Applications Availability Size Tested species Price Grade  
EB07478 Goat Anti-GFAP Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB07478 1
EB07478 (0.003µg/ml) staining of Human Cerebellum (A), (0.1ug/ml).Mouse (B) and (0.003µg/ml) Rat (C) Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07478 2
EB07478 (0.01µg/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary. This data is from a previous batch, not on sale.
EB07478 3
EB07478 (2µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. This data is from a previous batch, not on sale.
EB07478 4
EB07478 (5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
EB07478 5
EB07478 (5ug/ml) as the reporter with EB002015 as the capture rabbit antibody (5ug/ml). This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: GFAP, glial fibrillary acidic protein, FLJ45472, intermediate filament protein
Official Symbol: GFAP
Accession Number(s): NP_002046.1
Human GeneID(s): 2670
Non-Human GeneID(s): 14580 (mouse), 24387 (rat)
Important Comments: GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood, despite the number of studies using it as a cell marker.

Immunogen

Peptide with sequence C-DGEVIKESKQEHKD, from the C Terminus of the protein sequence according to NP_002046.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 48-50kDa band observed in Human Brain (Cerebellum), Mouse Brain and Rat Brain lysates (calculated MW of 49.9kDa according to Human NP_002046.1, Mouse NP_034407.2 and Rat NP_058705.2). Recommended concentration:0.003-0.01µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

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