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Goat Anti-Ghrelin preproprotein Antibody (EB06876)

Related Products: EBP06876 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB06876 Goat Anti-Ghrelin preproprotein Antibody Pep-ELISA, IHC, ICC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB06876 1
EB06876 (2µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ghrelin/obestatin, ghrelin, growth hormone secretagogue receptor ligand, OTTHUMP00000207798, OTTHUMP00000207796, OTTHUMP00000207795, OTTHUMP00000207794;, ghrelin/obestatin preprohormone, obestatin, ghrelin/obestatin prepropeptide, preproghrelin, motilin-related peptide, ghrelin precursor, ghrelin, MTLRP, GHRL
Official Symbol: GHRL
Accession Number(s): NP_057446.1; NP_001128413.1; NP_001128416.1; NP_001128417.1; NP_001128418.1
Human GeneID(s): 51738
Important Comments: This antibody is expected to recognise all reported isoforms.

Immunogen

Peptide with sequence C-QDILWEEAKEAPADK, from the C Terminus of the protein sequence according to NP_057446.1; NP_001128413.1; NP_001128416.1; NP_001128417.1; NP_001128418.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as small as the calculated size of this preproprotein of approx. 12.9kDa according to NP_057446.1 and of its derivatives. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Placenta shows staining of trophoblast cytoplasm. Recommended concentration, 2-5µg/ml.

ICC: This antibody has been successfully used in ICC for rat neurons as described in the following paper: Stoyanova et al, Regul Pept. 2009 Nov 27;158(1-3):86-90, PMID: 19540881.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human

Specific Reference

This antibody has been successfully used in the following papers:
Stoyanova II, Wiertz RW, Rutten WL.
Time-dependent changes in ghrelin-immunoreactivity in dissociated neuronal cultures of the newborn rat neocortex.
Regul Pept. 2009 Nov 27;158(1-3):86-90.
PMID: 19540881

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