Change country

Goat Anti-Ghrelin preproprotein Antibody (EB06876)

Code Name Applications Availability Size Tested species Price Grade  
EB06876 Goat Anti-Ghrelin preproprotein Antibody Pep-ELISA, IHC, ICC Yes 100µg specific antibody in 200µl Human, Rat $315.00 Elite

Ordering Information for

Order direct from Everest

$49.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

Order offline

Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB06876 1
EB06876 (2µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: GHRL, MTLRP, ghrelin, ghrelin precursor, motilin-related peptide, preproghrelin, ghrelin/obestatin prepropeptide, obestatin, ghrelin/obestatin preprohormone, OTTHUMP00000207794;, OTTHUMP00000207795, OTTHUMP00000207796, OTTHUMP00000207798, ghrelin, growth hormone secretagogue receptor ligand, ghrelin/obestatin
Official Symbol: GHRL
Accession Number(s): NP_057446.1; NP_001128413.1; NP_001128416.1; NP_001128417.1; NP_001128418.1
Human GeneID(s): 51738
Important Comments: This antibody is expected to recognise all reported isoforms.

Immunogen

Peptide with sequence C-QDILWEEAKEAPADK, from the C Terminus of the protein sequence according to NP_057446.1; NP_001128413.1; NP_001128416.1; NP_001128417.1; NP_001128418.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as small as the calculated size of this preproprotein of approx. 12.9kDa according to NP_057446.1 and of its derivatives. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Placenta shows staining of trophoblast cytoplasm. Recommended concentration, 2-5µg/ml.

Immunocytochemsitry: This antibody has been successfully used in ICC for rat neurons as described in the following paper: Stoyanova et al, Regul Pept. 2009 Nov 27;158(1-3):86-90, PMID: 19540881.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human

Specific Reference

This antibody has been successfully used in the following paper:
Stoyanova II, Wiertz RW, Rutten WL.
Time-dependent changes in ghrelin-immunoreactivity in dissociated neuronal cultures of the newborn rat neocortex.
Regul Pept. 2009 Nov 27;158(1-3):86-90.
PMID: 19540881

Product Reviews

Please login to review this product.