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Goat Anti-GLUT2 Antibody (EB09481)

Related Products: EBP09481 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09481 Goat Anti-GLUT2 Antibody Pep-ELISA, IHC, IF, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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$73.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09481 1
EB09481 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09481 2
EB09481 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09481 3
EB09481 (2.5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SLC2A2, solute carrier family 2 (facilitated glucose transporter), member 2, GLUT2
Official Symbol: SLC2A2
Accession Number(s): NP_000331.1
Human GeneID(s): 6514

Immunogen

Peptide with sequence C-RKEREEASSEQKVS, from the internal region of the protein sequence according to NP_000331.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
IHC: In paraffin embedded Human Liver shows strong staining of the endothelial lining cells. Recommended concentration: 2-6µg/ml.

Immunofluorescence: Strong expression of the protein seen in the membranes of HepG2 and in the membranes and nuclei of MCF7 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow, Pig

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