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Goat Anti-GPM6A Antibody (EB10558)

Related Products: EBP10558 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10558 Goat Anti-GPM6A Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10558 1
EB10558 (1µg/ml) staining of Human Cerebellum (A) and Olfactory Bulb (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10558 2
EB10558 (2µg/ml) staining of fetal Mouse (A) and Rat (B) Brain lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10558 3
EB10558 (3.75µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: GPM6A, glycoprotein M6A, GPM6, M6A, neuronal membrane glycoprotein M6-a
Official Symbol: GPM6A
Accession Number(s): NP_005268.1; NP_963885.1; NP_963886.1
Human GeneID(s): 2823
Non-Human GeneID(s): 234267 (mouse), 306439 (rat)
Important Comments: This antibody is expected to recognize all reported isoforms (NP_005268.1; NP_963885.1; NP_963886.1). Reported variants represent identical protein: NP_963885.1, NP_005268.1.

Immunogen

Peptide with sequence C-QTYFEMARTAGD, from the internal region of the protein sequence according to NP_005268.1; NP_963885.1; NP_963886.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 33kDa band observed in Human Brain (Cerebellum) and Rat Brain lysates. An approx. 75kDa band was also observed in Human Cerebellum, Olfactory Bulb and in fetal Mouse and Rat Brain lysates (calculated MW of 31.2kDa according to Human NP_005268.1 and Rat NP_835206.1, and 31.1kDa according to Mouse NP_705809.1). The observed 75kDa band corresponds to the glycosylated form. Recommended concentration: 0.5-2µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 3.75µg/ml.

This product has been demonstrated not suitable in IF.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

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