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Goat Anti-GPX4 (Isoform a and c) Antibody (EB07316)

Related Products: EBP07316 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB07316 Goat Anti-GPX4 (Isoform a and c) Antibody ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $300.00 Elite

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San Diego
CA 92121-4727
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Toll Free Tel: 1-800-550-3033
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EB07316 1
EB07316 (0.1µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07316 2
EB07316 (0.3µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: sperm nucleus glutathione peroxidase, phospholipid hydroperoxidase, glutathione peroxidase 4, snGPx, PHGPx, HGNC:4556, glutathione peroxidase 4 (phospholipid hydroperoxidase), GPX4
Official Symbol: GPX4
Accession Number(s): NP_002076.2; NP_001034937.1
Human GeneID(s): 2879
Important Comments: This antibody is expected to recognise isoform A (NP_002076.2) and isoform C (NP_001034937.1).

Immunogen

Peptide with sequence C-EEPLVIEKDLPHY, from the C Terminus of the protein sequence according to NP_002076.2; NP_001034937.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 19kDa band observed in Human Kidney and Testis lysates and in lysates of cell line HEK293 (calculated MW of 22.1kDa according to NP_002076.2). Recommended concentration: 0.1-0.3µg/ml.
IHC: In paraffin embedded Human Testis shows strong staining surrounding the nuclei of spermatocytes and surrounding spermatozoa. Recommended concentration, 0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Background Reference

Su D, Novoselov SV, Sun QA, Moustafa ME, Zhou Y, Oko R, Hatfield DL, Gladyshev VN.
Mammalian selenoprotein thioredoxin/glutathione reductase: Roles in disulfide bond formation and sperm maturation.
J Biol Chem. 2005 May 18; [Epub ahead of print]
PMID: 15901730

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