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Goat Anti-GPX4 (Isoform a and c) Antibody (EB07316)

Related Products: EBP07316 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB07316 Goat Anti-GPX4 (Isoform a and c) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig, Cow $315.00 Elite

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EB07316 1
EB07316 (0.1µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07316 2
EB07316 (0.3µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: sperm nucleus glutathione peroxidase, phospholipid hydroperoxidase, glutathione peroxidase 4, snGPx, PHGPx, HGNC:4556, glutathione peroxidase 4 (phospholipid hydroperoxidase), GPX4
Official Symbol: GPX4
Accession Number(s): NP_002076.2; NP_001034937.1
Human GeneID(s): 2879
Important Comments: This antibody is expected to recognise isoform A (NP_002076.2) and isoform C (NP_001034937.1).

Immunogen

Peptide with sequence C-EEPLVIEKDLPHY, from the C Terminus of the protein sequence according to NP_002076.2; NP_001034937.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 19kDa band observed in Human Kidney and Testis lysates and in lysates of cell line HEK293 (calculated MW of 22.1kDa according to NP_002076.2). Recommended concentration: 0.1-0.3µg/ml.
IHC: In paraffin embedded Human Testis shows strong staining surrounding the nuclei of spermatocytes and surrounding spermatozoa. Recommended concentration, 0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

Specific References

This antibody has been successfully used in the following papers:
Papp LV, Lu J, Bolderson E, Boucher D, Singh R, Holmgren A, Khanna KK.
SECIS-binding protein 2 promotes cell survival by protecting against oxidative stress.
Antioxid Redox Signal. 2010 Apr 1;12(7):797-808.
PMID: 19803747

Schoenmakers E et. al.
Mutations in the selenocysteine insertion sequence-binding protein 2 gene lead to a multisystem selenoprotein deficiency disorder in humans.
J Clin Invest. 2010 Dec 1;120(12):4220-35.
PMID: 21084748

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