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Goat Anti-GRB2, biotinylated Antibody (EB05284-B)

Code Name Applications Availability Size Tested species Price Grade  
EB05284-B Goat Anti-GRB2, biotinylated Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB05284-B 1
Biotinylated EB05284 (0.3µg/ml) staining of MOLT4 (A) and Human Thymus (B) lysates (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: GRB2, growth factor receptor-bound protein 2, ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2, HT027, SH2/SH3 adapter GRB2, abundant SRC homology, epidermal growth factor receptor-binding protein GRB2, growth factor receptor-bound protein 3, protein Ash
Official Symbol: GRB2
Accession Number(s): NP_002077.1; NP_987102.1
Human GeneID(s): 2885
Non-Human GeneID(s): 14784 (mouse), 81504 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).

Immunogen

Peptide with sequence C-PRNYVTPVNRNV., from the C Terminus of the protein sequence according to NP_002077.1; NP_987102.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 26kDa band observed in lysates of Human Thymus and of cell line MOLT4 (calculated MW of 25.2kDa according to NP_002077.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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