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Goat Anti-GRB7 (N Terminus) Antibody (EB05417)

Code Name Applications Availability Size Tested species Price Grade  
EB05417 Goat Anti-GRB7 (N Terminus) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB05417 1
EB05417 staining (2µg/ml) of A431 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB05417 2
HEK293 overexpressing GRB7 (RC215362) and probed with EB05417 (mock transfection in first lane), tested by Origene.
Click here to download PDF datasheet.

Target Protein

Principal Names: GRB7, growth factor receptor-bound protein 7, OTTHUMP00000164352
Official Symbol: GRB7
Accession Number(s): NP_005301.2
Human GeneID(s): 2886
Non-Human GeneID(s): 14786 (mouse)
Important Comments: This antibody recognises the longer isoform of this protein in A431 lysate.

Immunogen

Peptide with sequence ELDLSPPHLSSSPE-C, from the N Terminus of the protein sequence according to NP_005301.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60kDa band observed in lysates of cell line A431 (calculated MW of 59.7kDa according to NP_005301.2). In transfected HEK293 transiently expressing GRB7 a band of approx. 60kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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