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Goat Anti-Hdac1 (aa385-396) Antibody (EB11428)

Code Name Applications Availability Size Tested species Price Grade  
EB11428 Goat Anti-Hdac1 (aa385-396) Antibody Pep-ELISA, WB, ChIP Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB11428 1
ChIP of 2ug EB11428 with MCF7 chromatin using the Chromatrap® spin column sonication kit (Protein G) measuring H3 enrichment onto the p21 locus.
EB11428 2
EB11428 (0.3µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: HD1, Hdac1-ps, histone deacetylase 1, MGC102534, MGC118085, MommeD5, OTTMUSP00000010171, RP23-209C6.7, RPD3, HDAC1
Official Symbol: Hdac1
Accession Number(s): NP_032254.1
Human GeneID(s): 3065
Non-Human GeneID(s): 433759 (mouse), 84576 (rat)
Important Comments: This antibody may cross-react to HDAC2

Immunogen

Peptide with sequence C-PEDAIPEESGDE, from the internal region of the protein sequence according to NP_032254.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 55kDa band observed in lysates of cell line NIH3T3 (calculated MW of 55.1kDa according to Human NP_032254.1 and Mouse NP_032254.1). Recommended concentration: 0.3-1µg/ml.

Chromatin Immunoprecipitation: A 4-fold increased presence of H3 onto the Human P21 locus observed when comparing the precipitations using EB11428 with non-specific goat IgG to bring down HDAC1 in a chromatin lysate from MCF7 cells.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

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