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Goat Anti-HIP-55 / SH3P7 Antibody (EB05432)

Related Products: EBP05432 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB05432 Goat Anti-HIP-55 / SH3P7 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog $315.00 Elite

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EB05432 1
EB05432 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ABP1, drebrin-like, drebrin F, HIP-55 protein, src homology 3 domain-containing protein HIP-55, SH3P7, HIP-55, DBNL
Official Symbol: DBNL
Accession Number(s): NP_054782.2; NP_001014436.1; NP_001116428.1
Human GeneID(s): 28988
Non-Human GeneID(s): 13169 (mouse), 83527 (rat)
Important Comments: This antibody is expected to recognise all reported isoforms (NP_054782.2; NP_001014436.1; NP_001116428.1)

Immunogen

Peptide with sequence AANLSRNGPALQE-C, from the N Terminus of the protein sequence according to NP_054782.2; NP_001014436.1; NP_001116428.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55kDa band observed in Jurkat and 3T3 lysates (predicted MW of 49kDa according to NP_054782). Recommended for use at 1-3µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog

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