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Goat Anti-Histamine Receptor H2 Antibody (EB06905)

Code Name Applications Availability Size Tested species Price Grade  
EB06905 Goat Anti-Histamine Receptor H2 Antibody Pep-ELISA, IHC, IF Yes 100µg specific antibody in 200µl Rat $315.00 Elite

Ordering Information for

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

Target Protein

Principal Names: HRH2, histamine receptor H2, HGNC:5183, H2R, gastric receptor 1, OTTHUMP00000161242
Official Symbol: HRH2
Accession Number(s): NP_071640.1; NP_001124527.1
Human GeneID(s): 3274
Important Comments: This antibody is expected to recognise both reported isoforms.

Immunogen

Peptide with sequence C-QEEKPLKLQVWSGTE, from the C Terminus of the protein sequence according to NP_071640.1; NP_001124527.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
IHC: This product has been successfully used in IHC on Rat (PMID: 30226827).

Immunofluorescence:This product has been successfully used in IF on Rat (PMID: 28119568, 25633097, 23713466 and 23466693).

Species Reactivity

Tested: Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific References

This antibody has been successfully used in IHC on Rat:
Zhuang QX, Li GY, Li B, Zhang CZ, Zhang XY, Xi K, Li HZ, Wang JJ, Zhu JN.
Regularizing firing patterns of rat subthalamic neurons ameliorates parkinsonian motor deficits.
J Clin Invest. 2018 Sep 18.
PMID: 30226827

This antibody has been successfully used in IF on Rat:
Bin Li, Xiao-Yang Zhang, Ai-Hong Yang, Xiao-Chun Peng, Zhang-Peng Chen, Jia-Yuan Zhou, Ying-Shing Chan, Jian-Jun Wang and Jing-Ning Zhu.
Histamine Increases Neuronal Excitability and Sensitivity of the Lateral Vestibular Nucleus and Promotes Motor Behaviors via HCN Channel Coupled to H2 Receptor.
Front Cell Neurosci. 2017 Jan 10;10:300.
PMID: 28119568

This antibody has been successfully used in IF on Rat:
Zhang J, Zhuang QX, Li B, Wu GY, Yung WH, Zhu JN, Wang JJ.
Selective Modulation of Histaminergic Inputs on Projection Neurons of Cerebellum Rapidly Promotes Motor Coordination via HCN Channels.
Mol Neurobiol. 2016 Mar;53(2):1386-401.
PMID: 25633097

This antibody has been successfully used in IF on Rat:
Zhang XY, Yu L, Zhuang QX, Peng SY, Zhu JN, Wang JJ.
Postsynaptic mechanisms underlying the excitatory action of histamine on medial vestibular nucleus neurons in rats.
Br J Pharmacol. 2013 Sep;170(1):156-69.
PMID: 23713466

This antibody has been successfully used in IF on Rat:
Peng SY, Zhuang QX, He YC, Zhu JN, Wang JJ.
Histamine excites neurons of the inferior vestibular nucleus in rats by activation of H1 and H2 receptors.
Neurosci Lett. 2013 Apr 29;541:87-92.
PMID: 23466693

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