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Code Name Applications Availability Size Tested species Price Grade  
EB12204 Goat Anti-HOXA10 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB12204 1
EB12204 (0.2µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12204 2
EB12204 (0.3µg/ml) staining of Mouse Skeletal Muscle lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12204 3
EB12204 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: HOXA10, homeobox A10, HOX1, HOX1.8, HOX1H, PL, homeo box A10, homeobox protein 1H, homeobox protein HOXA10, homeobox protein Hox-1.8, homeobox protein Hox-1H, homeobox protein Hox-A10
Official Symbol: HOXA10
Accession Number(s): NP_061824.3
Human GeneID(s): 3206
Non-Human GeneID(s): 15395 (mouse)

Immunogen

Peptide with sequence PQATSCSFAQNIKE, from the internal region of the protein sequence according to NP_061824.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 37-38kDa band observed in Human and Mouse Skeletal Muscle lysates (calculated MW of 42.4kDa according to Human NP_061824.3 and 43.2kDa according to Mouse NP_032289.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: In paraffin embedded Human Testis shows nuclear staining preferentially in the spermatocytes and spermatids. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Pig, Cow

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