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Goat Anti-HP beta chain Antibody (EB11196)

Related Products: EBP11196 - Immunizing Peptide, EBP11197 - Immunizing Peptide, Goat Anti-HP alpha chain Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB11196 Goat Anti-HP beta chain Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Pig $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11196 1
EB11196 (0.1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11196 2
EB11196 (1µg/ml) staining of Pig Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: binding peptide, BP, haptoglobin, haptoglobin alpha(1S)-beta, haptoglobin alpha(2FS)-beta, haptoglobin, alpha polypeptide, haptoglobin, beta polypeptide, HP2ALPHA2, HPA1S, MGC111141, HP
Official Symbol: HP
Accession Number(s): NP_005134.1; NP_001119574.1
Human GeneID(s): 3240
Important Comments: This antibody is expected to recognize both reported isoforms (NP_005134.1; NP_001119574.1).

Immunogen

Peptide with sequence C-STVPEKKTPKSP, from the internal region of the protein sequence according to NP_005134.1; NP_001119574.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 37+40kDa bands observed in Human Liver lysates and 40kDa in Pig Liver lysates (calculated MW of 45.2kDa according to NP_005134.1 and 38.5kDa according to NP_001119574.1). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human, Pig
Expected from sequence similarity: Human, Dog, Pig

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