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Goat Anti-HRASLS3 / HREV107-3 Antibody (EB08338)

Code Name Applications Availability Size Tested species Price Grade  
EB08338 Goat Anti-HRASLS3 / HREV107-3 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB08338 1
EB08338 (5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PLA2G16, phospholipase A2, group XVI, AdPLA, H-REV107-1, HRASLS3, HREV107, HREV107-3, MGC118754, HRAS-like suppressor 3, adipose-specific PLA2
Official Symbol: PLAAT3
Accession Number(s): NP_009000.2
Human GeneID(s): 11145
Non-Human GeneID(s): 225845 (mouse), 24913 (rat)
Important Comments: Reported variants represent identical protein:NP_009000.2 and NP_001121675.1).

Immunogen

Peptide with sequence RAPIPEPKPGDLIE-C, from the N Terminus of the protein sequence according to NP_009000.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments gave an approx 28kDa band in Human Heart lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 18.0kDa according to NP_009000.1. The 28kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

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