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Goat Anti-HSPA4L Antibody (EB08505)

Related Products: EBP08505 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08505 Goat Anti-HSPA4L Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB08505 1
EB08505 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: HSPA4L, heat shock 70kDa protein 4-like, APG-1, Osp94, heat shock 70 kDa protein 4-like protein, heat shock protein (hsp110 family)
Official Symbol: HSPA4L
Accession Number(s): NP_055093.2
Human GeneID(s): 22824

Immunogen

Peptide with sequence C-DSSQHTKSSGEMEVD, from the C Terminus of the protein sequence according to NP_055093.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments gave an approx 125kDa band in Human Colon and Testis lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 94.5kDa according to NP_055093.2. The 125kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Testis. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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