Change country
Code Name Applications Availability Size Tested species Price Grade  
EB11753 Goat Anti-ICAM1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

Ordering Information for

Order direct from Everest

$49.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

Order offline

Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB11753 1
EB11753 (2µg/ml) staining of HeLa (A) and Daudi (B) cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11753 2
EB11753 (2µg/ml) staining of NIH3T3 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: BB2, CD54, cell surface glycoprotein P3.58, human rhinovirus receptor, ICAM1, ICAM-1, intercellular adhesion molecule 1, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, major group rhinovirus receptor, P3.58
Official Symbol: ICAM1
Accession Number(s): NP_000192.2
Human GeneID(s): 3383
Important Comments: The immunizing peptide represents part of the extracellular domain, and it does not overlap any known glycosite.

Immunogen

Peptide with sequence SNCPDGQSTAKT, from the internal region of the protein sequence according to NP_000192.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 100kDa band observed in lysates of cell lines Daudi, HeLa and NIH3T3 (calculated MW of 57.8kDa according to Human NP_000192.2 and 58.8kDa according to Mouse NP_034623.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pino et al, Reproduction. 2009 Nov;138(5):837-47. PMID: 19661147), and other commercial sources. Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human

Product Reviews

Please login to review this product.