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Goat Anti-IGFBP1 (aa156-170) Antibody (EB11949)

Related Products: EBP11949 - Immunizing Peptide, EBP11950 - Immunizing Peptide, Goat Anti-IGFBP1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB11949 Goat Anti-IGFBP1 (aa156-170) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11949 1
EB11949 (2µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11949 2
EB11949 (10µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: IGFBP1, insulin-like growth factor binding protein 1, AFBP, IBP1, IGF-BP25, PP12, hIGFBP-1, IBP-1, IGF-binding protein 1, IGFBP-1, OTTHUMP00000159561, OTTHUMP00000207990, OTTHUMP00000214348, alpha-pregnancy-associated endometrial globulin, amniotic fluid binding protein, binding protein-25, binding protein-26, binding protein-28, growth hormone independent-binding protein, insulin-like growth factor-binding protein 1, placental protein 12
Official Symbol: IGFBP1
Accession Number(s): NP_000587.1
Human GeneID(s): 3484

Immunogen

Peptide with sequence C-STYDGSKALHVTNIK, from the internal region of the protein sequence according to NP_000587.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 30kDa band observed in Human Placenta lysates (calculated MW of 27.9kDa according to NP_000587.1). Recommended concentration: 1-3µg/ml. An additional band of 55kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal.
IHC: Paraffin embedded Human Testis. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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