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|EB07784||Goat Anti-ILF2 / NF45 Antibody||Pep-ELISA, WB||100µg specific antibody in 200µl||Human||$315.00|
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Peptide with sequence C-EKAYEKPPEKKEG, from the internal region (near the C Terminus) of the protein sequence according to NP_004506.2; NP_001254738.1.
Please note the peptide is available for sale.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Immunoprecipitation: This antibody was confirmed by a customer in a special IP procedure called PAR-CLIP (see for method PMID: 20644507). This data is from a previous batch, not on sale.
This antibody has been successfully used in WB and IP on ESCs:
Ye J, Jin H, Pankov A, Song J, Blelloch R.
NF45 and NF90/NF110 coordinately regulate ESC pluripotency and differentiation
RNA. 2017 Aug;23(8):1270-1284.
This antibody has been successfully used in WB on Human:
Shamanna RA, Hoque M, Pe'ery T, Mathews MB.
Induction of p53, p21 and apoptosis by silencing the NF90/NF45 complex in human papilloma virus-transformed cervical carcinoma cells.
Oncogene. 2013 Oct 24;32(43):5176-85
This antibody (previous batch) has been successfully used in WB and IP on HeLa cells:
Shamanna RA, Hoque M, Lewis-Antes A, Azzam EI, Lagunoff D, Pe'ery T, Mathews MB.
The NF90/NF45 complex participates in DNA break repair via nonhomologous end joining.
Mol Cell Biol. 2011 Dec;31(23):4832-43.