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Goat Anti-ITCH / AIF4 Antibody (EB07645)

Related Products: EBP07645 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB07645 Goat Anti-ITCH / AIF4 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog $315.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are placed with and invoiced by our US partner Abcore LLC.

 
EB07645 1
EB07645 (0.03µg/ml) staining of Human Brain (Frontal Cortex) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ubiquitin protein ligase ITCH, itchy homolog E3 ubiquitin protein ligase, dJ468O1.1 (atrophin 1 interacting protein 4, AIP4), atrophin-1 interacting protein 4, NFE2-associated polypeptide 1, dJ468O1.1, NAPP1, AIP4, itchy homolog E3 ubiquitin protein ligase (mouse), AIF4, ITCH
Official Symbol: ITCH
Accession Number(s): NP_113671.3
Human GeneID(s): 83737
Non-Human GeneID(s): 16396 (mouse), 311567 (rat)

Immunogen

Peptide with sequence C-EIKSHDLKPNGGN, from the internal region of the protein sequence according to NP_113671.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 100kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 98.7kDa according to NP_113671.3). Recommended concentration: 0.03-0.1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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