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Goat Anti-ITGAM / CD11B Antibody (EB07825)

Code Name Applications Availability Size Tested species Price Grade  
EB07825 Goat Anti-ITGAM / CD11B Antibody Pep-ELISA, IF Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613

EB07825 1
EB07825 Immunofluorescence analysis of paraformaldehyde fixed U937 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
EB07825 2
EB07825 (2µg/ml) staining of paraffin embedded Human Brain. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: ITGAM, integrin, alpha M (complement component 3 receptor 3 subunit), CD11B, CR3A, MAC-1, MAC1A, MGC117044, MO1A, Integrin, alpha-M (complement component receptor-3, alpha), antigen CD11B (p170), macrophage antigen, alpha polypeptide, antigen CD11b (p170), complement component receptor 3, alpha, integrin alpha M, integrin, alpha M (complement component receptor 3, alpha), CD11b (p170), macrophage antigen alpha polypeptide, macrophage antigen alpha polypeptide, neutrophil adherence receptor alpha-M subunit
Official Symbol: ITGAM
Accession Number(s): NP_000623.2
Human GeneID(s): 3684
Non-Human GeneID(s): 16409 (mouse), 25021 (rat)
Important Comments: The immunizing peptide represents part of the extra-cellular domain.


Peptide with sequence C-RNDGEDSYRTQ, from the internal region of the protein sequence according to NP_000623.2 .

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U937 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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