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|EB05810||Goat Anti-KAP1 / TRIM28 Antibody||Pep-ELISA, IF, FC, IHC||100µg specific antibody in 200µl||Human||$315.00|
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Peptide with sequence C-SSQELSGGPGDGP, from the C Terminus of the protein sequence according to NP_005753.1.
Please note the peptide is available for sale.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Immunofluorescence: Strong expression of the protein seen in the nucleus of HeLa and U2OS cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
This antibody has been successfully used in ChIP in Human:
Frietze et al
ZNF274 recruits the histone methyltransferase SETDB1 to the 3' ends of ZNF genes.
PLoS One. 2010 Dec 8;5(12)
This antibody has been successfully used in IHC in Human:
Yokoe T, Toiyama Y, Okugawa Y, Tanaka K, Ohi M, Inoue Y, Mohri Y, Miki C, Kusunoki M.
KAP1 Is Associated With Peritoneal Carcinomatosis in Gastric Cancer.
Ann Surg Oncol. 2010 Mar;17(3):821-8.