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Goat Anti-KCNQ1 Antibody (EB09214)

Code Name Applications Availability Size Tested species Price Grade  
EB09214 Goat Anti-KCNQ1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB09214 1
EB09214 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: KCNQ1, potassium voltage-gated channel, KQT-like subfamily, member 1, ATFB1, FLJ26167, JLNS1, KCNA8, KCNA9, KVLQT1, Kv1.9, Kv7.1, LQT, LQT1, RWS, SQT2, WRS, Jervell and Lange-Nielsen syndrome 1, kidney and cardiac voltage dependend K+ channel, long (electrocardiographic) QT syndrome, Ward-Romano syndrome 1, slow delayed rectifier channel subunit
Official Symbol: KCNQ1
Accession Number(s): NP_000209.2; NP_861463.1
Human GeneID(s): 3784
Non-Human GeneID(s): 16535 (mouse), 84020 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_000209.2; NP_861463.1).

Immunogen

Peptide with sequence C-EQLTVPRRGPDEGS, from the C Terminus of the protein sequence according to NP_000209.2; NP_861463.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 60kDa band observed in Human Heart lysates (calculated MW of 61.5kDa according to NP_861463.1). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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