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Goat Anti-LAG3 / CD223 Antibody (EB13056)

Related Products: EBP07683 - Immunizing Peptide, EBP13056 - Immunizing Peptide, Goat Anti-LAG3 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB13056 Goat Anti-LAG3 / CD223 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB13056 1
EB13056 (1µg/ml) staining of Human Lymph Node (A) and (0.5ug/ml) Tonsil (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: LAG3, lymphocyte activating 3, CD223, lymphocyte-activation gene 3
Official Symbol: LAG3
Accession Number(s): NP_002277.4
Human GeneID(s): 3902
Important Comments: This antibody is expected to recognize the extra-cellular domain.

Immunogen

Peptide with sequence EDVSQAQAGTYTCH, from the internal region of the protein sequence according to NP_002277.4.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 55kDa band observed in Human Lymph Node and Tonsil lysates (calculated MW of 57.4kDa according to NP_002277.4). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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