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Goat Anti-Lamin B1 (aa526-537) Antibody (EB11655)

Code Name Applications Availability Size Tested species Price Grade  
EB11655 Goat Anti-Lamin B1 (aa526-537) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB11655 1
EB11655 (1µg/ml) staining of HeLa (A) and Jurkat (B) nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11655 2
EB11655 (1µg/ml) staining of Mouse Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ADLD, lamin B1, lamin-B1, LMN, LMN2, LMNB, LMNB1, MGC111419, OTTHUMP00000159218
Official Symbol: LMNB1
Accession Number(s): NP_005564.1; NP_001185486.1
Human GeneID(s): 4001
Non-Human GeneID(s): 16906 (mouse)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_005564.1; NP_001185486.1).

Immunogen

Peptide with sequence C-DVKVILKNSQGE, from the internal region (near C terminus) of the protein sequence according to NP_005564.1; NP_001185486.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 70kDa band observed in nuclear lysates of cell lines HeLa and Jurkat and approx. 70-75kDa in Mouse Liver lysates (calculated MW of 66.4kDa according to Human NP_005564.1 and 66.8kDa according to Mouse NP_034851.2) Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Dog, Pig, Cow

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