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Goat Anti-MEPI / SERPINI2 Antibody (EB06689)

Related Products: EBP06689 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB06689 Goat Anti-MEPI / SERPINI2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog $315.00 Elite

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EB06689 1
EB06689 (0.3µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06689 2
EB06689 (3µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining
Click here to download PDF datasheet.

Target Protein

Principal Names: serine (or cysteine) proteinase inhibitor, clade I (pancpin), member 2, protease inhibitor 14, myoepithelium-derived serine protease inhibitor, serpin peptidase inhibitor, clade I (pancpin), member 2, serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 2, SERPIN12, TSA2004, PANCPIN, PI14, MEPI, SERPINI2
Official Symbol: SERPINI2
Accession Number(s): NP_006208.1; NP_001012303.1
Human GeneID(s): 5276
Non-Human GeneID(s): 67931 (mouse), 171149 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_006208.1; NP_001012303.1).

Immunogen

Peptide with sequence C-KGDWKQKFRKEDTQ, from the internal region of the protein sequence according to NP_006208.1; NP_001012303.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 40kDa band observed in Human Pancreas lysates (calculated MW of 46.1kDa according to NP_006208.1 and 47.4kDa according to NP_001012303.1). Recommended concentration: 0.3-1µg/ml.
IHC: In paraffin embedded Human Pancreas, shows cytoplasmic staining of acinar cels and of rare cells in the islets of Langerhans. Recommended concentration, 3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific Reference

This antibody has been successfully used in the following papers:
Wehr AY, Furth EE, Sangar V, Blair IA, Yu KH.
Analysis of the human pancreatic stellate cell secreted proteome.
Pancreas. 2011 May;40(4):557-66.
PMID: 21499210

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