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Goat Anti-MKRN1 (aa105-118) Antibody (EB10898)

Related Products: EBP06756 - Immunizing Peptide, EBP10898 - Immunizing Peptide, Goat Anti-Makorin Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10898 Goat Anti-MKRN1 (aa105-118) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10898 1
EB10898 (1µg/ml) staining of Mouse fetal (A) and adult (B) Brain lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB10898 2
HEK293 lysate (10ug protein in RIPA buffer) over expressing Human MKRN1 with DYKDDDDK tag probed with EB10898 (0.3ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB10898 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: MKRN1, makorin ring finger protein 1, FLJ21334, RNF61, E3 ubiquitin-protein ligase makorin-1, OTTHUMP00000212121, OTTHUMP00000212124, RING finger protein 61
Official Symbol: MKRN1
Accession Number(s): NP_038474.2; NP_001138597.1
Human GeneID(s): 23608
Non-Human GeneID(s): 54484 (mouse)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_038474.2; NP_001138597.1).

Immunogen

Peptide with sequence C-RYEHSKPLKQEEAT, from the internet regoin of the protein sequence according to NP_038474.2; NP_001138597.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 50kDa band observed in fetal Mouse Brain lysates and 35kDa plus fainter 50kDa bands in adult Mouse Brain lysates (calculated MW of 52.9kDa according to NP_061280.2). The observed 35kDa band corresponds to the calculated MW of human isoform 2 (35.2kDa according to NP_001138597.1), but this size isoform has not yet been confirmed in Mouse. Recommended concentration: 1-3µg/ml. In transfected HEK293 transiently expressing full-length Human MKRN1 (myc and DYKDDDDK tagged), a band of approx. 60kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 0.3- 1µg/ml.

Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email [email protected] to request datasheet, pricing and delivery information.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Dog, Pig, Cow

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