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Goat Anti-MMP3 (aa459-473) Antibody (EB11719)

Code Name Applications Availability Size Tested species Price Grade  
EB11719 Goat Anti-MMP3 (aa459-473) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB11719 1
EB11719 (0.3µg/ml) staining of Mouse Eye lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11719 2
EB11719 (0.2µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CHDS6, matrix metallopeptidase 3 (stromelysin 1, progelatinase), matrix metalloproteinase 3 (stromelysin 1, progelatinase), matrix metalloproteinase-3, MGC126102, MGC126103, MGC126104, MMP3, MMP-3, OTTHUMP00000045949, proteoglycan, SL-1, STMY, STMY1, STR1
Official Symbol: MMP3
Accession Number(s): NP_002413.1
Human GeneID(s): 4314

Immunogen

Peptide with sequence C-DPNAKKVTHTLKSNS, from the C Terminus of the protein sequence according to NP_002413.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 55kDa band observed in Human Prostate and approx. 60kDa band in Mouse Eye lysates (calculated MW of 54.0 kDa according to Human NP_002413.1 and Mouse NP_034939.1). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identity was also consistently observed at 24kDa in Human. This band was successfully blocked by incubation with the immunizing peptide.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human

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