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Goat Anti-MPG Antibody (EB06330)

Related Products: EBP06330 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB06330 Goat Anti-MPG Antibody ELISA, WB Yes 100µg specific antibody in 200µl Human $300.00 Elite

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EB06330 1
EB06330 staining (1µg/ml) of HEK293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: proliferation-inducing protein 16, proliferation-inducing protein 11, alkyladenine DNA glycosylase, CRA36.1 (3-methyl-adenine DNA glycosylase), 3\1 end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on 16p, anpg, PIG16, PIG11, N-methylpurine-DNA glycosylase, MPG, Mid1, MDG, CRA36.1, APNG, AAG, N-methylpurine-DNA glycosylase
Official Symbol: MPG
Accession Number(s): NP_002425.2; NP_001015052.1; NP_001015054.1
Human GeneID(s): 4350
Important Comments: This antibody is expected to recognize all three reported isoforms (NP_002425.2, NP_001015052.1 and NP_001015054.1)

Immunogen

Peptide with sequence C-SVVDRVAEQDTQA, from the C Terminus of the protein sequence according to NP_002425.2; NP_001015052.1; NP_001015054.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 37kDa band observed in lysates of HEK293 and K562 (calculated MW of 32.9kDa according to NP_002425.2). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Background Reference

Samson L, Derfler B, Boosalis M, Call K.
Cloning and characterization of a 3-methyladenine DNA glycosylase cDNA from human cells whose gene maps to chromosome 16.
Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9127-31.
PMID: 1924375

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