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Goat Anti-MTM1 Antibody (EB05195)

Related Products: EBP05195 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB05195 Goat Anti-MTM1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Dog, Cow $315.00 Elite

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EB05195 1
HEK293 overexpressing MTM1 (RC205306) and probed with EB05195 (mock transfection in first lane), tested by Origene.
EB05195 2
EB05195 (0.3µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: myotubularin 1, myotubularin, XLMTM, MTMX, CNM, myotubular myopathy 1, MTM1
Official Symbol: MTM1
Accession Number(s): NP_000243.1
Human GeneID(s): 4534

Immunogen

Peptide with sequence C-SSPSQMMPHVQTHF, from the C Terminus of the protein sequence according to NP_000243.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 70kDa band observed in lysates of Human Muscle and of cell line HepG2 (calculated MW of 69.9kDa according to NP_000243.1). In transfected HEK293 transiently expressing MTM1 a band of approx. 65kDa is observed. This band is not observed in the non-transfected

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog, Cow

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