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Goat Anti-MURF1 / TRIM63 (N Term) Antibody (EB05879)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB05879 | Goat Anti-MURF1 / TRIM63 (N Term) Antibody | Pep-ELISA, IHC |  |
100µg specific antibody in 200µl | Human, Dog, Cow | $315.00 |  |
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$40.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be processed and invoiced by our US partner Abcore LLC. Order offlineNow shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:
In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day. Orders are placed with and invoiced by our US partner Abcore LLC.
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Target Protein
Principal Names: muscle specific ring finger protein 1, tripartite motif-containing 63, striated muscle RING zinc finger protein, muscle specific ring finger protein 2, iris ring finger protein, FLJ32380, MURF2, SMRZ, IRF, ring finger protein 28, RNF28, MURF-1, MURF1, TRIM63Official Symbol: TRIM63
Accession Number(s): NP_115977.2
Human GeneID(s): 84676
Immunogen
Peptide with sequence DYKSSLIQDGNPM-C, from the N Terminus of the protein sequence according to NP_115977.2.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Preliminary experiments gave an approx 60kDa band in Human Skeletal Muscle lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.2kDa according to NP_115977.2. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Skeletal Muscle shows strong staining in rare nuclei, and weak staining of muscle fibres in longitudinal section.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Dog, Cow
Specific References
The goat polyclonal antibody used in this paper was manufactured by us:
Salanova M, Schiffl G, Püttmann B, Schoser BG, Blottner D.
Molecular biomarkers monitoring human skeletal muscle fibres and microvasculature following long-term bed rest with and without countermeasures.
J Anat. 2008 Mar;212(3):306-18.
PMID: 18221329
The goat polyclonal antibody used in this paper was manufactured by us:
de Palma L, Marinelli M, Pavan M, Orazi A.
Ubiquitin ligases MuRF1 and MAFbx in human skeletal muscle atrophy.
Joint Bone Spine. 2008 Jan;75(1):53-7.
PMID: 17977773
The goat polyclonal antibody used in this paper was manufactured by us:
Urso ML, Chen YW, Scrimgeour AG, Lee PC, Lee KF, Clarkson PM.
Alterations in mRNA expression and protein products following spinal cord injury in humans.
J Physiol. 2007 Mar 15;579(Pt 3):877-92.
PMID: 17218363